SrasV1, Main, Exploration, bibRecord, 006029

Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.

Identifieur interne : 006029 ( Main/Exploration ); précédent : 006028; suivant : 006030

Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.

Auteurs : Yuling Shi [République populaire de Chine] ; Yanping Yi ; Ping Li ; Tieji Kuang ; Linhai Li ; Mei Dong ; Qingjun Ma ; Cheng Cao

Source :

Journal of clinical microbiology [ 0095-1137 ] ; 2003.

RBID : pubmed:14662982

Descripteurs français

KwdFr :
- Amorces ADN, Anticorps antiviraux, Humains, Nucléocapside (génétique), Nucléocapside (immunologie), Réaction de polymérisation en chaîne, Syndrome respiratoire aigu sévère (diagnostic), Séquence nucléotidique, Test ELISA (), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Nucléocapside, Virus du SRAS.
- immunologie : Nucléocapside, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Amorces ADN, Anticorps antiviraux, Humains, Réaction de polymérisation en chaîne, Séquence nucléotidique, Test ELISA.

English descriptors

KwdEn :
- Antibodies, Viral, Base Sequence, DNA Primers, Enzyme-Linked Immunosorbent Assay (methods), Humans, Nucleocapsid (genetics), Nucleocapsid (immunology), Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis).
MESH :
- chemical : Antibodies, Viral, DNA Primers.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : Nucleocapsid, SARS Virus.
- immunology : Nucleocapsid, SARS Virus.
- isolation & purification : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Base Sequence, Humans, Polymerase Chain Reaction.

Abstract

Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.

DOI: 10.1128/jcm.41.12.5781-5782.2003
PubMed: 14662982

Affiliations:

Le document en format XML

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<term>Humans</term>
<term>Nucleocapsid (genetics)</term>
<term>Nucleocapsid (immunology)</term>
<term>Polymerase Chain Reaction</term>
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<term>SARS Virus (immunology)</term>
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<front><div type="abstract" xml:lang="en">Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.</div>
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Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.

Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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